Cracking the AP Biology Exam

15

Laboratory

LAB 5: CELL RESPIRATION

In this lab, the respiratory rate of germinating and nongerminating (dry) peas was investigated. The equation for cellular respiration is:

C6H12O6 + 6O2 → 6CO2 + 12H2O + energy (ATP)

Germinating peas respire and need to consume oxygen in order to continue to grow. Nongerminating pea seeds do not respire actively. In this lab, the amount of oxygen consume was measured using a respirometer. The experiment was also conducted at two temperatures, 25° C and 10° C, because peas consume more oxygen at higher temperatures.

What are the concepts that you need to know?

  • Oxygen is consumed in cellular respiration.
  • Germinating peas have a higher respiratory rate while nongerminating peas have a low respiratory rate.
  • Know how to design a study to determine the affect of temperature on cell respiration.
  • Be able to explain the significance of a control using glass beads.